NPT anticorps

N° du produit ABIN932234

L’anticorps Souris Monoclonal anti- a été validé pour ELISA et WB. Il convient pour détecter dans des échantillons de Humain.

Aperçu rapide pour NPT anticorps (ABIN932234)

Antigène: NPT

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB)

Clone: 4B4D1

Détail du produit

Immunogène: NPT antibody was raised in Mouse using a purified recombinant fragment of NPT expressed in E. coli as the immunogen.

Isotype: IgG2a

Information d'application

Indications d'application: ELISA: 1:10,000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in ascitic fluid containing 0.03 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4 °C for short term storage. Store at -20 °C for long term storage.

Détail du antigène

Antigène: NPT

Sujet: NPT is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPT is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents. Synonyms: Monoclonal NPT antibody, Anti-NPT antibody, Neomycin phosphotransferase II antibody, NptII antibody.